human gastric mucosal epithelial cell line  (ATCC)

Journal: World Journal of Gastrointestinal Oncology

Article Title: Interleukin-34 promotes the proliferation and epithelial-mesenchymal transition of gastric cancer cells

doi: 10.4251/wjgo.v14.i10.1968

Figure Lengend Snippet: Expression of interleukin-34 in gastric cancer tissues and cell lines, and construction of AGS cell lines with stable knockdown or overexpression of interleukin-34. A: Representative immunohistochemistry (IHC) staining of interleukin (IL)-34 in adjacent normal tissue, gastric cancer (GC) tissues, (scale bar = 25 μm); B: IHC staining scores were used to evaluate IL-34 expression in GC tissues and adjacent normal tissue; C: Western blotting was used to detect IL-34 protein expression in gastric normal epithelial cells (GES-1) and GC cell lines (AGS, HGC-27, and MKN-45); D: The relative densitometric analysis of protein bands was calculated; E: IL-34 mRNA expression was detected by quantitative real-time polymerase chain reaction (qRT-PCR); F and G: Western blotting was used to verify the downregulation of IL-34 in AGS cell lines; H: qRT-PCR was used to verify the downregulation of IL-34 in AGS cell lines; I and J: Western blotting was used to verify the overexpression of IL-34 in AGS cell lines; K: qRT-PCR was used to verify the overexpression of IL-34 in AGS cell lines. b P < 0.01, c P < 0.001.

Article Snippet: The human normal gastric mucosal epithelial cell line (GES-1) and human GC cell lines (AGS, MKN-45 and HGC-27) were provided by Prof. Aman Xu (The First Affiliated Hospital of Anhui Medical University).

Techniques: Expressing, Knockdown, Over Expression, Immunohistochemistry, Western Blot, Real-time Polymerase Chain Reaction, Quantitative RT-PCR

Journal: World Journal of Gastrointestinal Oncology

Article Title: Interleukin-34 promotes the proliferation and epithelial-mesenchymal transition of gastric cancer cells

doi: 10.4251/wjgo.v14.i10.1968

Figure Lengend Snippet: Interleukin-34 regulates the expression of epithelial-mesenchymal transition-related proteins in AGS cells. A-D: Downregulation of endogenous interleukin (IL)-34 increases E-cadherin expression, and reduces the expression of N-cadherin and vimentin in AGS cells; E-H: Upregulation of endogenous IL-34 reduces the E-cadherin expression, but increases the expression of N-cadherin and vimentin in AGS cells. Data was experiments performed in triplicate and expressed as mean ± standard deviation. b P < 0.01.

Article Snippet: The human normal gastric mucosal epithelial cell line (GES-1) and human GC cell lines (AGS, MKN-45 and HGC-27) were provided by Prof. Aman Xu (The First Affiliated Hospital of Anhui Medical University).

Techniques: Expressing, Standard Deviation